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KMID : 0378019750180091181
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New Medical Journal
1975 Volume.18 No. 9 p.1181 ~ p.1188
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The Experimental Study of the Articular Cartilage after Synovectomy and Cutting the Cruciate Ligaments
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Abstract
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Mature articular cartilage is an aneural, avascular, and alymphatic specialized form of tissue 1, 2. 6, 9, 12, 14, 15, 16, 20, 25, :0). The initial degeneration of articular cartilage may occur spontaneously with normal wear and tear in aging cartilage, or it may occur in articular cartilage that has been damaged by the local conditions, such as injury or synovectomy. It is the purpose of this report to study the ultrastructural and light microscopical appearance of the articular cartilage after synovectomy and cutting the cruciate ligaments of rabbits.
Thirty young rabbits each weighing about 2.0kg were used for the experimental study and the animals were sacrificed at time intervals of 3, 7,14.28, and 42 days respectively after the synovectomy or cutting the cruciate ligaments. The tissue were decalcified and prepared by standard paraffin embedding procedure and stained with hematoxylin and eosin for histologic observation.
For electron microscopy, the specimen fixed in 4% glutaraldehyde for 4 hours and postfixed for 2 hours at 4°C with 1% osmium tetraoxide in 0. 1 M phosphate buffer with pH 7.426). All tissues were dehydrated in graded alcohol and embedded in Epon 812 according to standard procedures. They were cut into section of 500 A with a glass knife. The sections were mounted on copper grids, stained with uranyl acetate and lead hydroxide and observed with the Hitachi 11-E Electron Microscope.
The results of the light and electron microscopic findings are as follows;
1. The degeneratve changes of the articular cartilage were appeared after 7 days and became increasingly severe in later samples in the groups of synovectomy. But the degenerative changes of the articular cartilage obtained 6 weeks after synovectomy in light microscope were not severe.
2. The degenerative changes of the articular cartilage were appeared after 7 days and became increasingly severe in later samples in the group of the cutting the cruciate ligaments.
3. By the electron microscopic findings, the degenerative changes of the articular cartilage were appeared after 1 week and continued steadily up to 6 weeks in the group of synovectomy. And the degenerative changes of the articular cartilage in the group of the cutting the cruciate ligaments were severe in degree comparing to the group of synovectomy.
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